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The Class II KNOX gene KNAT7 negatively regulates secondary wall formation in Arabidopsis and is functionally conserved in Populus.

Identifieur interne : 002892 ( Main/Exploration ); précédent : 002891; suivant : 002893

The Class II KNOX gene KNAT7 negatively regulates secondary wall formation in Arabidopsis and is functionally conserved in Populus.

Auteurs : Eryang Li [Canada] ; Apurva Bhargava ; Weiya Qiang ; Michael C. Friedmann ; Natascha Forneris ; Rodney A. Savidge ; Lee A. Johnson ; Shawn D. Mansfield ; Brian E. Ellis ; Carl J. Douglas

Source :

RBID : pubmed:22236040

Descripteurs français

English descriptors

Abstract

• The formation of secondary cell walls in cell types such as tracheary elements and fibers is a defining characteristic of vascular plants. The Arabidopsis transcription factor KNAT7 is a component of a transcription network that regulates secondary cell wall biosynthesis, but its function has remained unclear. • We conducted anatomical, biochemical and molecular phenotypic analyses of Arabidopsis knat7 loss-of-function alleles, KNAT7 over-expression lines and knat7 lines expressing poplar KNAT7. • KNAT7 was strongly expressed in concert with secondary wall formation in Arabidopsis and poplar. Arabidopsis knat7 loss-of-function alleles exhibited irregular xylem phenotypes, but also showed increased secondary cell wall thickness in fibers. Increased commitment to secondary cell wall biosynthesis was accompanied by increased lignin content and elevated expression of secondary cell wall biosynthetic genes. KNAT7 over-expression resulted in thinner interfascicular fiber cell walls. • Taken together with data demonstrating that KNAT7 is a transcriptional repressor, we hypothesize that KNAT7 is a negative regulator of secondary wall biosynthesis, and functions in a negative feedback loop that represses metabolically inappropriate commitment to secondary wall formation, thereby maintaining metabolic homeostasis. The conservation of the KNAT7 regulatory module in poplar suggests new ways to manipulate secondary cell wall deposition for improvement of bioenergy traits in this tree.

DOI: 10.1111/j.1469-8137.2011.04016.x
PubMed: 22236040


Affiliations:


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Le document en format XML

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<term>Arabidopsis (cytology)</term>
<term>Arabidopsis (genetics)</term>
<term>Arabidopsis Proteins (genetics)</term>
<term>Arabidopsis Proteins (metabolism)</term>
<term>Cell Nucleus (metabolism)</term>
<term>Cell Wall (metabolism)</term>
<term>Cell Wall (ultrastructure)</term>
<term>Conserved Sequence (MeSH)</term>
<term>Gene Expression Profiling (MeSH)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Genes, Plant (genetics)</term>
<term>Genetic Complementation Test (MeSH)</term>
<term>Glucuronidase (metabolism)</term>
<term>Green Fluorescent Proteins (metabolism)</term>
<term>Inflorescence (metabolism)</term>
<term>Inflorescence (ultrastructure)</term>
<term>Lignin (metabolism)</term>
<term>Mutagenesis, Insertional (genetics)</term>
<term>Mutation (genetics)</term>
<term>Phenotype (MeSH)</term>
<term>Phylogeny (MeSH)</term>
<term>Plant Stems (anatomy & histology)</term>
<term>Plant Stems (metabolism)</term>
<term>Plant Stems (ultrastructure)</term>
<term>Plants, Genetically Modified (MeSH)</term>
<term>Populus (genetics)</term>
<term>Protein Transport (MeSH)</term>
<term>Real-Time Polymerase Chain Reaction (MeSH)</term>
<term>Repressor Proteins (genetics)</term>
<term>Repressor Proteins (metabolism)</term>
<term>Sequence Homology, Amino Acid (MeSH)</term>
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<term>Analyse de profil d'expression de gènes (MeSH)</term>
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<term>Arabidopsis (génétique)</term>
<term>Glucuronidase (métabolisme)</term>
<term>Gènes de plante (génétique)</term>
<term>Inflorescence (métabolisme)</term>
<term>Inflorescence (ultrastructure)</term>
<term>Lignine (métabolisme)</term>
<term>Mutagenèse par insertion (génétique)</term>
<term>Mutation (génétique)</term>
<term>Noyau de la cellule (métabolisme)</term>
<term>Paroi cellulaire (métabolisme)</term>
<term>Paroi cellulaire (ultrastructure)</term>
<term>Phylogenèse (MeSH)</term>
<term>Phénotype (MeSH)</term>
<term>Populus (génétique)</term>
<term>Protéines d'Arabidopsis (génétique)</term>
<term>Protéines d'Arabidopsis (métabolisme)</term>
<term>Protéines de répression (génétique)</term>
<term>Protéines de répression (métabolisme)</term>
<term>Protéines à fluorescence verte (métabolisme)</term>
<term>Réaction de polymérisation en chaine en temps réel (MeSH)</term>
<term>Régulation de l'expression des gènes végétaux (MeSH)</term>
<term>Similitude de séquences d'acides aminés (MeSH)</term>
<term>Séquence conservée (MeSH)</term>
<term>Test de complémentation (MeSH)</term>
<term>Tiges de plante (anatomie et histologie)</term>
<term>Tiges de plante (métabolisme)</term>
<term>Tiges de plante (ultrastructure)</term>
<term>Transport des protéines (MeSH)</term>
<term>Végétaux génétiquement modifiés (MeSH)</term>
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<term>Arabidopsis Proteins</term>
<term>Repressor Proteins</term>
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<term>Tiges de plante</term>
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<term>Plant Stems</term>
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<term>Arabidopsis</term>
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<term>Arabidopsis</term>
<term>Gènes de plante</term>
<term>Mutagenèse par insertion</term>
<term>Mutation</term>
<term>Populus</term>
<term>Protéines d'Arabidopsis</term>
<term>Protéines de répression</term>
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<term>Arabidopsis Proteins</term>
<term>Cell Nucleus</term>
<term>Cell Wall</term>
<term>Glucuronidase</term>
<term>Green Fluorescent Proteins</term>
<term>Inflorescence</term>
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<term>Repressor Proteins</term>
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<term>Lignine</term>
<term>Noyau de la cellule</term>
<term>Paroi cellulaire</term>
<term>Protéines d'Arabidopsis</term>
<term>Protéines de répression</term>
<term>Protéines à fluorescence verte</term>
<term>Tiges de plante</term>
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<term>Cell Wall</term>
<term>Inflorescence</term>
<term>Plant Stems</term>
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<term>Alleles</term>
<term>Conserved Sequence</term>
<term>Gene Expression Profiling</term>
<term>Gene Expression Regulation, Plant</term>
<term>Genetic Complementation Test</term>
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<term>Phylogeny</term>
<term>Plants, Genetically Modified</term>
<term>Protein Transport</term>
<term>Real-Time Polymerase Chain Reaction</term>
<term>Sequence Homology, Amino Acid</term>
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<div type="abstract" xml:lang="en">• The formation of secondary cell walls in cell types such as tracheary elements and fibers is a defining characteristic of vascular plants. The Arabidopsis transcription factor KNAT7 is a component of a transcription network that regulates secondary cell wall biosynthesis, but its function has remained unclear. • We conducted anatomical, biochemical and molecular phenotypic analyses of Arabidopsis knat7 loss-of-function alleles, KNAT7 over-expression lines and knat7 lines expressing poplar KNAT7. • KNAT7 was strongly expressed in concert with secondary wall formation in Arabidopsis and poplar. Arabidopsis knat7 loss-of-function alleles exhibited irregular xylem phenotypes, but also showed increased secondary cell wall thickness in fibers. Increased commitment to secondary cell wall biosynthesis was accompanied by increased lignin content and elevated expression of secondary cell wall biosynthetic genes. KNAT7 over-expression resulted in thinner interfascicular fiber cell walls. • Taken together with data demonstrating that KNAT7 is a transcriptional repressor, we hypothesize that KNAT7 is a negative regulator of secondary wall biosynthesis, and functions in a negative feedback loop that represses metabolically inappropriate commitment to secondary wall formation, thereby maintaining metabolic homeostasis. The conservation of the KNAT7 regulatory module in poplar suggests new ways to manipulate secondary cell wall deposition for improvement of bioenergy traits in this tree.</div>
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<AbstractText>• The formation of secondary cell walls in cell types such as tracheary elements and fibers is a defining characteristic of vascular plants. The Arabidopsis transcription factor KNAT7 is a component of a transcription network that regulates secondary cell wall biosynthesis, but its function has remained unclear. • We conducted anatomical, biochemical and molecular phenotypic analyses of Arabidopsis knat7 loss-of-function alleles, KNAT7 over-expression lines and knat7 lines expressing poplar KNAT7. • KNAT7 was strongly expressed in concert with secondary wall formation in Arabidopsis and poplar. Arabidopsis knat7 loss-of-function alleles exhibited irregular xylem phenotypes, but also showed increased secondary cell wall thickness in fibers. Increased commitment to secondary cell wall biosynthesis was accompanied by increased lignin content and elevated expression of secondary cell wall biosynthetic genes. KNAT7 over-expression resulted in thinner interfascicular fiber cell walls. • Taken together with data demonstrating that KNAT7 is a transcriptional repressor, we hypothesize that KNAT7 is a negative regulator of secondary wall biosynthesis, and functions in a negative feedback loop that represses metabolically inappropriate commitment to secondary wall formation, thereby maintaining metabolic homeostasis. The conservation of the KNAT7 regulatory module in poplar suggests new ways to manipulate secondary cell wall deposition for improvement of bioenergy traits in this tree.</AbstractText>
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